Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion.

Publication Type:

Journal Article

Source:

PloS one, Volume 7, Issue 1, p.e30219 (2012)

Keywords:

Actins, CARCINOMA, Consortium Authored Paper, Cytoskeletal Proteins, DISEASE PROGRESSION, Epithelial-Mesenchymal Transition, Fibroblasts, Gene Expression Regulation, Neoplastic, Humans, Models, Biological, Myofibroblasts, Neoplasm Invasiveness, Neoplasms, Pancreatic Neoplasms, Phosphoproteins, Proteomics Core Facility, Shared Resources, Stromal Cells, Tumor Cells, Cultured

Abstract:

Cancer-associated fibroblasts, comprised of activated fibroblasts or myofibroblasts, are found in the stroma surrounding solid tumors. These myofibroblasts promote invasion and metastasis of cancer cells. Mechanisms regulating the activation of the fibroblasts and the initiation of invasive tumorigenesis are of great interest. Upregulation of the cytoskeletal protein, palladin, has been detected in the stromal myofibroblasts surrounding many solid cancers and in expression screens for genes involved in invasion. Using a pancreatic cancer model, we investigated the functional consequence of overexpression of exogenous palladin in normal fibroblasts in vitro and its effect on the early stages of tumor invasion.