Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae.

Publication Type:

Journal Article

Source:

Genetics, Volume 183, Issue 4, p.1591-5 (2009)

Keywords:

2009, Amino Acid Sequence, Basic Sciences Division, Center-Authored Paper, Genomics Core Facility, Intracellular Signaling Peptides and Proteins, Kinetochores, Microtubules, Mutation, Nuclear Proteins, PHOSPHORYLATION, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Shared Resources

Abstract:

Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.