Allele-Specific PCR for Determination of IL28B Genotype.

Publication Type:

Journal Article


Journal of clinical microbiology, Volume 50, Issue 12, p.4144-6 (2012)


2012, Center-Authored Paper, Genomics Core Facility, October 2012, Shared Resources, Vaccine and Infectious Disease Division


IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.