Advancing the High Throughput Identification of Disease Specific Protein Signatures Using Multiplexed Ion Mobility Spectrometry.

Publication Type:

Journal Article

Source:

Molecular & cellular proteomics : MCP (2014)

Keywords:

2014, January 2014, Vaccine and Infectious Disease Division

Abstract:

Rapid diagnosis of disease states using less invasive, safer, and more clinically acceptable approaches than presently employed is a crucial direction for the field of medicine. While mass spectrometry (MS)-based proteomics approaches have attempted to meet these objectives, challenges such as the enormous dynamic range of protein concentrations in clinically relevant biofluid samples coupled with the need to address human biodiversity have slowed their employment. Herein, we report on the use of a new instrumental platform that addresses these challenges by coupling technical advances in rapid gas phase multiplexed ion mobility spectrometry (IMS) separations with liquid chromatography (LC) and MS to dramatically increase measurement sensitivity and throughput, further enabling future high throughput MS-based clinical applications. An initial application of the LC-IMS-MS platform analyzing blood serum samples from 60 post-liver transplant patients with recurrent fibrosis progression and 60 non-transplant patients illustrates its potential utility for disease characterization.