Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV.

Publication Type:

Journal Article

Source:

Journal of virology, Volume 84, Issue 15, p.7908-10 (2010)

Keywords:

2010, Animals, Basic Sciences Division, Cell Line, Tumor, Cell Transformation, Viral, Center-Authored Paper, Endogenous Retroviruses, Genes, ras, Genes, Viral, Genome, Viral, Genomics Core Facility, Human Biology Division, Humans, Rats, Shared Resources

Abstract:

Virus from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) can induce rare foci of transformation in rat 208F fibroblasts. Characterization of three such foci revealed that one produced an acutely transforming virus at a high titer. The virus consists of a mutant Nras cDNA from the HT-1080 cells inserted into a retroviral vector (added to the HT-1080 cells as a marker for infection) in place of internal vector sequences. These results show that XMRV can generate acutely transforming viruses at a low rate, as is typical of other replication-competent retroviruses, and reveal the potential for transforming virus contamination of retroviral vectors made from transformed cell lines.