Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD.

Publication Type:

Journal Article

Source:

Proceedings of the National Academy of Sciences of the United States of America, Volume 86, Issue 14, p.5434-8 (1989)

Keywords:

1989, Adipose Tissue, Animals, Cell Differentiation, Cell Line, Fibroblasts, Gene Expression Regulation, Genes, Regulator, Genetic Vectors, Humans, liver, MELANOMA, Moloney murine sarcoma virus, Muscles, Neuroblastoma, Transfection

Abstract:

MyoD is a master regulatory gene for myogenesis. Under the control of a retroviral long terminal repeat, MyoD was expressed in a variety of differentiated cell types by using either a DNA transfection vector or a retrovirus. Expression of muscle-specific proteins was observed in chicken, human, and rat primary fibroblasts and in differentiated melanoma, neuroblastoma, liver, and adipocyte lines. The ability of MyoD to activate muscle genes in a variety of differentiated cell lines suggests that no additional tissue-specific factors other than MyoD are needed to activate the downstream program for terminal muscle differentiation or that, if such factors exist, they are themselves activated by MyoD expression.