Absolute quantification by droplet digital PCR versus analog real-time PCR.

Publication Type:

Journal Article


Nature methods, Volume 10, Issue 10, p.1003-5 (2013)


2013, Clinical Research Division, Human Biology Division, Public Health Sciences Division, September 2013


Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37-86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.