RNA (Canis Lupus Familiaris)

Contact: Michael A. Harkey, Ph.D.
Contact phone: (206) 667-3369
Contact fax: (206) 667-5978
Contact e-mail: mharkey@fredhutch.org

Total RNA is extracted from tissues of normal healthy animals. Each tissue is Snap-Frozen in Liquid Nitrogen (LN), milled to a fine powder under LN in a freezer mill, and extracted with Trizol Reagent. mRNA is further purified by silica-binding on Qiagen RNAeasy columns. Purity and integrety of the RNA is tested by NanoDrop spectrophotometry, capillary electrophoresis, and RTPCR of a 1.7 Kb Beta Actin region.

Included in your sample will be:

  • 100 ug total RNA, frozen on dry ice.
  • Description of tissue source, including age, sex, health of donor, and results of quality control assays (see example)

RNAs from the following tissues are presently available:

  • Brain
  • Skin
  • Liver
  • Bone marrow
  • Spleen
  • Blood
  • Heart
  • Testis
  • Kidney
  • Uterus
  • Lung

How to Acquire Product

For extramural colleagues access to all reagents and SOPs requires execution of a Fred Hutch UBMTA and a nominal fee that covers production and mailing. Please fill out the UBMTA and email it to mharkey@fredhutch.org. If you need something not listed here, contact us. We may have it.

See our rates.