MHC Class-I Allele Tetramers

Contact: Jianhong Cao, Ph.D., Staff Scientist and Director
Contact phone: (206) 667-6455
Contact fax: (206) 667-6845
Contact e-mail: jcao@fredhutch.org

Immune Monitoring has a collection of MHC tetramers that they have previously produced and are available for purchase. Currently, we have 2 mouse and 22 human alleles available for tetramer production (Table 1). Tetramers for other species are also available. Contact the resource for more information. We offer tetramers conjugated with PE, APC, AlexaFluor 647 and Qdot.

Table 1. Available MHC Class-I alleles for tetramer production

Human Murine
A*0101
A*0201
A*0201 M (A245V)
A*0201 DM (D227K/T228/A)
A*0301
A*1101
A*2301
A*24021
A*2501
A*2902
A*3002
A*3101
A*68012
B*0702
B*0801
B*1402
B*1501
B*1801
B*3501
B*3502
B*4001
B*5101
B*5201
Cw*0101
Cw*0202
Cw*0701
Cw*0702
Cw*1203
HLA-Eg
HLA-Er
HLA-F
HLA-G
H-2D(b)
H-2K(b)


Note: Individual tetramers can differ significantly in refolding, stability and staining of T cells. The differences are determined largely by the HLA alleles and the binding affinity of the specific peptide to the HLA molecule. We recommend the testing of each new batch of tetramers with positive and negative control cells and by titration of the tetramer reagent. Tetramers should be stored at 4°C and retested after long-term storage. Please visit the NIH Tetramer Core Facility's website for more information.

How to acquire product:

For any of the above tetrameres please use iLab to request the service.