Canine cDNA and Reporter Constructs

Contact: Michael A. Harkey, Ph.D.
Contact phone: (206) 667-3369
Contact fax: (206) 667-5978
Contact e-mail: mharkey@fredhutch.org

One type of product of the CCEH Cell and Molecular Reagents facility includes cDNA expression and reporter vectors for the Canis lupis familiaris model.

cDNA expression constructs: Full-length cDNA clones are available for several genes expressed in pluripotent cells of the inner cell mass of the canine blastocyst. These are lenti-viral constructs designed by Dr. Aravind Ramakrishnan, at Fred Hutch, for the purpose of generating canine induced pluripotent stem (ips) cells. Each cDNA was cloned from a blastocyst cDNA library (see our library page), and verified by sequencing, expression and Western blot analysis.

Presently available cDNA vectors:

  • CMV-cOct3/4
  • CMV-cSox2
  • CMV-cMyc
  • CMVcKLF4.

Reporter constructs: A lenti-viral construct in which the canine Oct3/4 promoter drives expression of fluorescent reporter, YFP, was developed to track generation of canine ips cells. Additional constructs, using promoters from canine and human Sox2, Klf4, and Nanog are under development and will be available when verified.

Presently available reporter vectors:

  • cOct3/4-YFP

Future constructs: Additional canine cDNA and reporter constructs will be added as they are developed and/or acquired from other sources, and verified.


How to Acquire Product

For extramural colleagues access to all reagents and SOPs requires execution of a Fred Hutch UBMTA and a nominal fee that covers production and mailing. Please fill out the MTA and email it to mharkey@fredhutch.org. If you need something not listed here, contact us. We may have it.

See our rates.