Updated Instrumentation and Training Opportunities in Scientific Imaging

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Tuesday 08/06/2013

New Zeiss LSM 780 confocal and multiphoton microscope

The Zeiss 780 is equipped with five lasers, providing seven excitation lines at 405nm, 458nm, 488nm, 514 nm, 561nm, 594nm, and 633. The instrument also includes a novel, filter-less spectral detection system that can be freely tuned for any fluorescent dye or protein emitting in the visible range (400-700 nm), including nuclear stains such as DAPI. The Gallium-Arsenide detectors of the LSM 780 offer approximately twice the detection efficiency of conventional photomultipliers. All in all, the LSM 780 is one of the most sensitive and versatile confocal microscopes currently available. 

The instrument is controlled by Zeiss ZEN software, which allows the acquisition of single confocal images, confocal stacks, time-lapse experiments, multi-point, and large image acquisition (automated image stitching). The instrument also allows photobleaching (FRAP), photoactivation, and fluorescence resonance energy transfer (FRET) experiments.

Finally, the instrument is fitted with a state of the art multiphoton laser (Coherent Chameleon Vision II) that can be tuned from 680 nm to 1030 nm. Coupled with a set of very sensitive non-descanned detectors, two photon microscopy is useful for imaging UV-excited dyes (such as NADH), as well as conventional dyes and fluorescent proteins at greater depth than with confocal imaging. Researchers not familiar with two photon imaging should consult with the Resource to see if this imaging mode might benefit them.

Updated Volocity and Imaris 3-D visualization and analysis software

The Imaris suite is one of the most powerful software packages available for visualization and analysis of 3-D microscopy data. We recently upgraded our Imaris suite to the latest version, capable of running on the latest operating systems. Up-to-date versions of Volocity software are also available.  Other software available in the Resource includes the Cellomics high content analyze suite, Tissue Quest and Histoquest software for the analysis of histology data, Metamorph, ImageJ, and more.

New high performance workstation for image visualization and analysis

We have acquired three high performance computers (two 64-bit Windows PCs, and one 64-bit Mac workstation). These powerful workstations allow visualization and analysis of large 3-D microscopy data sets (including image stacks from the confocal or deconvolution microscopes, and 3-D time-lapse data). 

Wealth of Resources in New Scientific Imaging LibGuide 

A guide for Scientific Imaging has been added to the collection of LibGuides hosted by the Arnold Library. The guide provides a wealth of references, books, websites and other useful information for all Scientific Imaging resource users. It can be accessed at http://libguides.fhcrc.org/scientific_imaging

Microscopy Class: Introduction to Microscopy and Image Analysis

The Scientific Imaging resource is planning the second installment of its introductory class on microscopy and image analysis in the Fall of 2013.  For more information, please refer to the resource's website or email imaging@fhcrc.org.

Other instruments and services available

Scientific Imaging provides a broad range of instrumentation and imaging techniques, including true confocal (point scanning), spinning disk and swept field confocal for live imaging, total internal reflection (TIRF) and super resolution microscopy (N-STORM), 3-D deconvolution, time lapse and live cell imaging (including stage top incubators for a variety of cell culture vessels), high content screening (plate imaging) and whole slide imaging (for high resolution imaging of tissue sections, cell smears, and tissue micro array (TMA) slides).  The Typhoon and Odyssey scanners and dedicated software (Odyssey, ImageQuant) are also available for gel and blot imaging and quantitative analysis.  Researchers are always encouraged to contact the Resource manager to discuss current or future needs, and make suggestions or requests for new instrumentation. 

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Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources