Nikon TIRF System

Friday 06/03/2011

The new Nikon total internal reflection fluorescence (TIRF) microscope is designed to provide outstanding signal to noise when imaging samples right at the coverslip surface. TIRF uses a special illumination system to limit fluorescence excitation to a thin sheet of a few hundred nanometers, thereby reducing or elimination most background fluorescence signal.

This system is specially recommended for imaging weak fluorescent signals at the cell surface (such as membrane signaling events, cell-cell adhesion, etc...). The system is also ideal for single molecule imaging, such as tracking motor proteins along reconstituted microtubules in vitro.