NextGen Sequencing

Related Core Facilities:

Monday 06/13/2011

NextGen sequencing by lane is now available on our Illumina HiSeq 2000.  Researchers can register for 1 or more lanes of SR50, SR100, or PE50 sequencing runs.  Sequencing will proceed when all 8 lanes of a flow cell are reserved. 

For more information and details on how to reserve sequencing lanes, please visit http://genomics.fhcrc.org/grdp2/ngs/Announcement.html  or contact the Genomics Resource (see below).

NextGen sequencing library prep and QC services are now available. 

Services include:

  • RNA/DNA QC, Covaris fragmentation* (when applicable),
  • library preparation using standard Illumina TruSeq kits,
  • additional QC (Agilent Bioanalyzer*),
  • and final template concentration estimation and QC using Agilent’s qPCR NGS Concentration Quantification kit*. 

* Services are available separately for a fee.

For details please contact the Genomics Resource:
Andy Marty (email: jamarty@fhcrc.org; ph: 206-667-1393)
Jeff Delrow (email: jdelrow@fhcrc.org;  ph: 206-667-2763)
 

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Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources