New Instruments and Clinical Grade Tetramers in Immune Monitoring

Related Core Facilities:

Sunday 09/23/2012

xCelligence System

The xCelligence system measures changes in electrode impedance caused by cells on top of the electrodes. It can be used to monitor cell viability, number, morphology, and adhesion degree in a number of cell-based assays.

Clinical Grade Tetramers

Immune Monitoring has started producing clinical grade pMHC Class-I tetramers. These custom-made, sterile and highly purified products are used to sort antigen-specific CD8+ T cells, which are cultured and then used for immune therapy trials.  The tetramers are approved by the FDA to use in certain IND protocols at the Center.

Synergy H4 Hybrid Multi-Mode Microplate Reader

The Synergy H4 reader has Quadruple monochromator-based optics and filters which provide flexibility in usage.  Any wavelength can be selected by users from the low UV to the near infrared. Detection methods include fluorescence, time-resolved fluorescence, fluorescence polarization, AlphaScreen/AlphaLISA, luminescence, and UV-visible absorbance. Read methods include end point, kinetic, spectral scanning, and well-area scanning. It can read various plate formats, from 6-well to 384-well plates.

For assistance with any of the above, please contact Jianhong Cao at cao@fhcrc.orgor x6455.

Cytokine Analysis

The Cytokine Lab has now moved under Immune Monitoring. We provide access to equipment for state of the art immunoassays including Luminex and ELISA assays. Any questions can be directed to Rick Lawler at

rlawler@fhcrc.orgor x5235.

Contact David Hockenberry at dhockenb@fhcrc.orgfor questions about Seahorse XF24 Cellular Bioenergetics.