New Instrumentation and Instrument Updates

Related Core Facilities:

Tuesday 09/25/2012

DeltaVision Elite

Scientific Imaging acquired an Applied Precision DeltaVision Elite microscope system to replace our old DeltaVision RT. The new instrument uses a novel solid state illumination engine instead of the more traditional mercury arc or metal halide fluorescence lamp. The new illuminator bypasses the need for excitation filters, allowing for very fast switching between fluorescence channels. Also, since the new light engine does not produce harmful UV light, the system is ideally suited for live cell imaging.

A proprietary light delivery system provides intense and even illumination of the field of view, resulting in up to tenfold increase in brightness and dramatically reduced exposure times, as well as enhanced image contrast due to reduced scattered light.  The DeltaVision Elite is also equipped with a hardware focus tracking system, and an environmental chamber for temperature controlled experiments. This is a superb microscope system for high resolution imaging of thin live or fixed specimens (yeasts, cultured cells, thin tissue sections, & worm embryos).

Nikon TIRF & Super Resolution (STORM) Microscope

A Nikon Total Internal Reflection Fluorescence (TIRF)  microscope is now operational. TIRF is ideally suited for the visualization of faint signals close to the coverslip surface, including single molecule detection, and is recommended for the study of protein dynamics at or near the cell membrane, cell-cell adhesion, and the visualization of biomolecules in in vitro systems (such as microtubule assembly and molecular motors).

The TIRF system is also equipped with the hardware and software required for super resolution imaging using     Nikon's N-STORM localization microscopy technology.     N-STORM allows precise localization of individual molecules labeled with a suitable fluorescent dye pair, and can achieve resolutions down to approximately 20 nm, ten times better than conventional microscopy. Researchers interested in utilizing this technology should contact the Resource for additional information.

New Photoactivation/FRAP Device on Nikon Live Swept Field Confocal Microscope

Our Nikon/Prairie Technologies swept field confocal system (Nikon Live) has a new photoactivation device attachment. The device was custom-built by Prairie Technologies, and allows photobleaching, photoactivation, and cell ablation experiments while imaging with the swept field system. The FRAP device includes 405 and 488 nm lasers for conventional photoactivation/photoconversion/photobleaching experiments, as well as a high power UV laser (375 nm) for special applications (such as DNA damage and cell ablation). The imaging and photobleaching are controlled through PrairieView software.

Scientific Imaging Plans to Acquire the Following   Instruments in 2012-2013

We plan to obtain a new state-of-the-art Laser Scanning Confocal microscope (late 2012)  to complement and/or replace our existing Zeiss LSM 510. Several instruments are under consideration, including the Zeiss LSM 710 and 780, and the Leica SP8. The new confocal will offer greater functionality, sensitivity, convenience, as well as new capabilities. 

An upgrade of the Cellomics ArrayScan plate screening platform is planned. It will include a new light engine for fluorescence excitation to improve image quality and versatility, and a new user-friendly software interface that simplifies experiment design and assay optimization.

Additionally, Scientific Imaging will purchase at least two new high performance imaging computers that will increase the stability and speed of our high-end imaging software, and facilitate visualization and analysis of large images and data sets generated by current instruments.

New Rates for Scientific Imaging

The Scientific Imaging Resource has revised the rates for its instruments. The new rates are designed to balance the costs between instruments, and reduce costs for extended time lapse experiments lasting six hours or longer. Please contact the Resource for details.

Contact the Resource manager, Julio Vazquez at imaging@fhcrc.orgor x4205 to discuss current or future needs, and make suggestions for new instrumentation.

 

image description

Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources