New and Improved Method to OCT Embedded Tissue

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Saturday 08/01/2009

Looking for an easier way to embed your tissue in OCT for frozen sections? Experimental Histopathology has a new safe, easy, and economical system for you!

There are many research applications for tissue that has not been fixed or processed through paraffin including Laser Capture Microdiscection and protein and nucleic acid analysis. In addition, some immunohistochemistry and special stains protocols require frozen sections. In all of these cases, excellent tissue morphology is required. For that reason, tissue must be frozen in a media such as OCT under controlled conditions. Freezing tissue directly in liquid nitrogen or too slowly in a cryostat can lead to significant tissue damage and artifact. The preferred method involves freezing OCT embedded tissue in an isopentane bath over liquid nitrogen. However, isopentane can be dangerous, expensive, and difficult to work with in the lab or necropsy room.

Experimental Histopathology’s latest protocol for freezing tissue in OCT eliminates the need for isopentane and allows you to freeze more specimens in less time. You will be able to produce high quality tissue morphology in blocks that are easier to cut.

Please contact Experimental Histopathology for questions about OCT embedding (call 206-667-6166 or stop by at DE-360). We also cut and stain frozen sections for LCM, H&E, special stains, and IHC.

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Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources