Mass Spectrometry-based Proteomics

Related Core Facilities:

Monday 07/30/2018

Mass Spectrometry-based Proteomics will be held on September 24th – 28th, 2018 from 2:00 pm – 5:00 pm daily. The instructor will be Phil Gafken, PhD; Director, Proteomics Facility.

The course is recommended for Fred Hutch staff who are currently utilizing proteomics or who plan to use proteomics in the near future. The course is intended to educate novice users of the technology to the level where they have a solid understanding of it and they could apply the gained knowledge to their research. 

Class is free to Fred Hutch employees. Space is limited. Anyone interested in taking the course should register by email with Elizabeth Shumway, eshumway@fredhutch.org.

TOPICS COVERED

  • Basics of Mass Spectrometers
  • Data Acquisition; LC/MS
  • Protein Identification and Quantification
  • Characterizing Post-translational Modifications
  • Analysis of Complex Mixtures
  • Targeted Proteomics
  • Basis for Database Search Algorithms
  • Rudimentary Analysis of Proteomics Data
  • Preparing Samples for Mass Spectrometry
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Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources
Director, Comparative Medicine