Laser Capture Microdissection (LCM) Instrumentation and Services

Related Core Facilities:

Friday 06/03/2011

Due to the heterogeneous nature of tissue, it is often difficult to generate useful genetic and protein data from whole tissues.  Laser Capture Microdissection (LCM) is a method that allows for the separation or isolation of specific cells from tissue. The power of this technique is that LCM procedure does not alter or damage the morphology or the genetic and protein content of the captured cells or the surrounding cells. This technique can be useful for many applications including the comparison of tumor cells to surrounding non-tumor cells.

LCM can be performed on a number of tissue samples including blood smears, cytology preparations, frozen tissue, and formalin-fixed, paraffin-embedded tissue samples. Keep in mind: While the LCM process does not change the chemistry of the sample, fixation, processing and handling can. Please consult Experimental Histopathology staff before you collect your tissue samples.

Equipment:  Arcturus "Veritas" Laser Capture Microdissection System

Services include:

Training on LCM equipment
• Pre-LCM processing of samples including OCT embedding or paraffin-processing
• LCM sectioning including RNase free sectioning
• Histological staining to visualize tissue, including RNase free conditions
• Reference H&E sectioning and staining