Fix and Decal All in One leads to Better Whole Head Sections

Saturday 08/01/2009

Collaborating, Experimental Histopathology and Veterinary Pathology, have developed new protocols that allow you to fix and decalcify bone samples, including whole mouse heads, in one easy step.

Don’t lose your head! The head is an important part of the mouse.  In just five coronal sections, you can examine the mouse from snout to cerebellum.  This technique allows visualization of the following structures, as well as associated structures that may be important for accurate diagnosis and phenotype characterization: Cerebellum (Section 1), Ear/Pituitary (Section 2), Cerebrum (Section 3), Eyes (Section 4), and Nose (Section 5). This technique allows visualization of the brain without the artifacts that can occur at necropsy.  It also allows you to visualize brain tumors in vivo.  In addition to coronal sections, we can also now produce high quality paraffin sections of horizontal and sagital orientations.

To support these studies, we have also developed modified immunohistochemistry protocols for Ki67, BrdU, and Cleaved Caspase 3. Experimental Histopathology can adapt other IHC protocols as required.

These techniques can also be used when evaluating other bone specimens such as marrow cores, femurs, and ribs.

Please consult with Sue Knoblaugh or Julie Randolph-Habecker; for the best protocol to meet your experimental needs.

In addition, Scientific Imaging now has a microscope for scanning slides called Tissuefaxs that can be used visualize the entire section of the head. 
 

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Shared Resources are core facilities that provide services and access to specialized equipment for research activities.


Rajesh K. Uthamanthil, DVM, PhD, DACLAM

Associate Vice President, Shared Resources