Experimental Histopathology sponsoring monthly National Society for Histotechnology Webinar Series

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Tuesday 12/31/2013

Experimental Histopathology is sponsoring the National Society for Histotechnology Webinar Series in 2014. Webinars will be held the 4th Wednesday of each month from 10:00-11:00 a.m. in D5-110. Details about each webinar are available below. Contact Julie Randolph-Habecker for additional information. 


Wednesday, January 22

Immuno-Staining Cytologic Specimen Material: Practical Considerations and Potential Pitfalls
Presented by: Joseph Myers, CT(ASCP)QIHC, Biocare Medical, Clearwater, FL

This webinar is intended to provide participants with a comprehensive overview of the procedures involved in immune-staining cytologic (i.e., loosely cellular) specimen material – including smears, fine-needle aspirates, Cytospin ™ preparations, “monolayer” preparations, and cell blocks prepared using traditional and newly developed methods/devices. Although fundamentally similar to immune-staining histologic (i.e., tissue) specimen, the primary differences in processing and handling cytologic specimens will be emphasized. Since one of the most important differences in immune-staining cytologic material is the use of “non-aldehyde” fixatives (which requires use of different methods of compensating), this talk will include a discussion on heat-induced epitope retrieval (HIER). In a similar manner, since staining cytologic “test” specimens requires use of similarly handled control material, methods of creating cytologic control samples will also be discussed. Handouts, including reference tables and sample protocols, will be provided.


Wednesday, February 26

Pre-analytical Variables important to Modern Surgical Pathology: Increasing the clinical and research potential of the paraffin block
Presented by: Cecilia Yeung, MD, Fred Hutchinson Cancer Research Center, Seattle, Washington

Elastin fibers stretch up to 1.5 times their length and easily return to their original lengthwhen relaxed. They allow for movements and prevent rigidity throughout the human body. When problems arise with elasticity, there can be serious health risks for patients. It is diagnostically important to determine or rule out a complication relating to elastin fibers. There are many special stains for elastin fibers. In this presentation, we shall cover the special stains that are specific for elastin (VVG, Aldehyde fuchsin, Orcein, Miller, ResorcinFuchsin), how they work, and troubleshooting. Topics related to disease process and the important role of elastin fibers will also be discussed.


Wednesday, March 26

UFOs (UnFamiliar Objects) on Slides
Presented by: Elizabeth Sheppard, HT(ASCP), Ventana Medical Systems, Tucson, AZ

Artifacts are an inescapable facet of life in the histology laboratory. Often they are only a time consuming annoyance, but artifacts can have important technical and even medico legal implications. It is just as important to prevent, identify and solve these mysteries when they arise. In this webinar we will investigate three broad categories: technical errors in processing, embedding, sectioning and staining; artifacts of diagnostic significance; and miscellaneous artifacts. The participant will gain the ability to identify unfamiliar objects and learn techniques to avoid them.


Wednesday, April 23

Immunohistochemistry Staining for Orphan Metastatic Tumors (Metastasis from Unknown Primary)
Presented by: Mitual Amin, MD, Beaumont Hospital, Royal Oak, MI

Tumors from their primary/origin sites often metastasize to other tissues and organs in the body, and in some instances this may be the first clinical presentation of this tumor. In such cases where a biopsy of the metastasis is presented to a pathologist, the histologic features are usually diagnostic or they may need a little support with the help of immunohistochemical stains to help ascertain the site of primary. In rare instances, tumors appear undifferentiated offering no clue as to the primary origin of these tumors and these cases can be rather challenging. Assessing the site of primary is critical to the management of these patients. In such cases, immunohistochemistry plays a central role in assessing the nature and site of primary origin of this tumor. Discussed in this presentation will be a somewhat simplified approach as to how pathologists may order certain panels of antibodies and their assessments. This will help participants become aware of the existence of such tumors, and the utility and limitations of these immunohistochemical tests in this setting.


Wednesday, May 28

Emerging and Re-Emerging Infectious Disease and Zoonoses
Presented by: Richard French, DVM, PhD, Becker College, Worcester, MA

The interaction between human and animal health is not a new phenomenon. However, the scope, scale, and world-wide impact of zoonoses we are facing today are unprecedented. During the past 20 years, at least 30 new diseases have emerged, for many of which there is no treatment, cure or vaccine, or the possibility of effective prevention or control. In addition, the uncontrolled and inappropriate use of antibiotics has resulted in increased antimicrobial resistance and is seriously threatening drug control strategies against such common diseases as tuberculosis, malaria, cholera, dysentery and pneumonia. The unprecedented impact of globalization, industrialization, restructuring of agricultural systems and consumerism, among others, have certainly had an impact. Also of significance and with numerous examples, world climate changes are playing a role. Most of the recent emerging diseases have an animal origin, and almost all of them have zoonotic potential. These diseases must therefore be addressed through coordinated actions between animal and public health authorities. This webinar covers issue and recent findings on a number of emerging diseases and disease outbreaks. Where do you live?


Wednesday, June 25

MICROWAVES: Microwaves: Make You Want to Go Hmmm?
Presented by: Donna Willis, HT(ASCP)HTL, Baylor University Medical Center, Dallas, TX

Do you ever questions about how a microwave works? Does it really process tissue faster than conventional processors? What tests (stains, IHC) can I perform using microwave technology? How do I validate microwave equipment? What do I do when something goes wrong? Oh right, nothing ever goes wrong in Histology. In this webinar all these questions will be answered. At the end of the sessions, participants will be able to determine why they would consider using a microwave in their laboratory. They will also be able to troubleshoot the problems that can occur while using microwave technology. Time for questions to be answered will be allowed at the end of the session.


Wednesday, July 23

Rapid and Efficient Tissue Processing with Microwave Technology
Presented by: Zoe Ann Durkin, HT(ASCP), LabPulse Medical, Easy Granby, CT

This webinar will relate the fast and easy use of microwave technology to process tissue without the use of xylene as the clearing agent. Participants will review fixation factors, reagents used in tissue processing, concepts and schedules. This presentation incorporates the use of a microwave for processing anything from rush biopsies to routine work in a reduced time frame and in some cases, the potential of eliminating longer overnight runs. A comparison of microwave processing and traditional tissue processing schedules will be made demonstrating how microwaves have the ability to speed up processing times in the histology laboratory without the loss of quality.


Wednesday, August 20

Let’s talk about...Skin! How to Optimize the Grossing, Processing and H&E Staining of Skin Biopsies
Presented by: Nancy Warren, HT(ASCP)SLS, Truman Medical Center Hospital Hill, Kansas City, MO and Garth R. Fraga, MD, University of Kansas Medical Center, Kansas City, KS

Not all skin is the same. Working with various skin types is not only skin beauty business, but it is also Histology business. In this webinar, we will discuss how best to “gross in” different types of skin specimens, and offer tips on optimizing the processing, embedding, and microtomy of skin biopsies. We will also discuss what properties in the hematoxylin-eosin stain are particularly desirable to dermatopathologists.


Wednesday, September 24

Histotechs in Motion: Evaluating Ergonomic Concerns for Your Laboratory
Presented by: Dale Telgenhoff, PhD, HTL (ASCP)CM, Tarleton State University, Fort Worth, TX

Histology laboratory employees are very familiar with health and safety issues related to chemical and biological exposure, and have extensive safety programs in place to monitor these areas. Ergonomic safety is included in these programs, but is usually a secondary consideration. According to the Occupational Safety and Health Administration (OSHA), repetitive motion injuries are the nation’s most common and costly occupational health problem. Repetitive strain injuries (RSIs) and cumulative trauma disorders (CTDs) are common in laboratory areas which require an over-use or misuse of muscles, tendons, and nerves. For the histotechnician, this includes microtomy, working in fume hoods and computer workstations, microscopy, and pipetting. Microtomy is an area of particular concern, as the average technician uses between 40 and 50 cassettes per shift, turning the microtome wheel at least 1000 times in an 8 hour period. In this webinar, we will discuss the causes of work related musculoskeletal disorders, the symptoms, risk factors, and strategies to avoid long-term injuries. Additionally we will discuss how to perform an ergonomic assessment in your laboratory to identify and rectify potential problem areas.


Wednesday, October 22

Principles of Perinatal Autopsy
Presented by: Jacqueline Macknis, MD, Beaumont Hospital, Royal Oak, MI

Histotechs are involved in embedding, sectioning and staining placenta and tissue from perinatal autopsies. This session will provide an overview of the principles of perinatal autopsy. We will discuss how perinatal autopsy can be a valuable tool in not only deciphering cause of death, but possibly uncovering etiologies that may have ramifications for future pregnancies. Examples of numerous congenital anomalies, inherited syndromes, and genetic disorders will be discussed. Basic dissection technique and normal histology will be reviewed. As examination of the placenta is a key aspect of any perinatal death, a brief overview of placental pathology will also be provided.


Wednesday, November 26

What Happened To My IHC & What Caused It?
Presented by: Jesse DelCampo, HT(ASCP), Biocare, Concord, CA

Wouldn’t it be great to be able to narrow down and solve most of our IHC stain issues by the tell-tale signs on our slides. Brown halo, light blue halo around the tissue sample, counterstain coming off purple instead of blue, cells within the tissue sample looks like clouds and does not even match the H&E anymore are some of the tell-tale signs that makes the most of us wonder. Let’s track it down to the source by understanding which steps causes what during the stain.


Wednesday, December 17

Human Epidermal Growth Factor Receptor 2 (HER2) Testing - Validation, Application and Correlation
Presented by: Joelle Weaver,MS,HTL(ASCP)QIHC, Louisville, KY

Human epidermal growth factor receptor 2 gene (ERBB2) commonly referred to as HER2 is amplified in 18% to 20 % of breast cancers. HER2 overexpression in invasive breast cancer is associated with a higher rate of recurrence and mortality. HER2 status is also predictive for several therapies. The optimal assay performance parameters to assess HER2 status, either by immunohistochemistry, in situ hybridization or fluorescent in situ hybridization, remains controversial. Testing accuracy and method validation remain a troubling issue. What validation and quality assurance strategies can your laboratory use to help ensure.