Electron Microscopy Equipment

Location: Thomas Building, DE-780
Contact phone: (206) 667-4289
Contact e-mail: emsr@fredhutch.org

JEOL JSM 6610LV Scanning EM

This microscope is a high-performance SEM for fast characterization and imaging of fine structures on both small and large samples. It is equipped with a large specimen chamber and automated specimen stage.

The microscope can be operated in low vacuum mode for observation of non-conductive specimens. A highly sensitive backscattered detector and low vacuum secondary detector are used for observation of these non-conductive specimens.

It offers a magnification range from 5X to 300,000X and has a resolution of 3.0nm at 30KV in the high vacuum mode.

JEOL JEM 1400 Transmission EM

The JEOL JEM-1400 Transmission Electron Microscope is a 120 KV instrument with a magnification range of 50X - 2,000,000X. The resolution is 0.20nm (lattice image).

JEOL JEM 1400 It is equipped with two Gatan CCD cameras. The side mounted Orius Gatan camera is an 11 megapixel camera that allows for rapid viewing and for applications requiring a large field of view. The bottom mount Gatan Ultrascan 1000XP camera is a 2K x 2K high resolution camera that also offers TV rate imaging.

This microscope also comes equipped with electron tomography software. The Dual-Axis Tomography holder by Fischione Instruments is for transmission electron microscopy imaging or analysis that requires in situ specimen rotation.

Electron tomography is a technique that can be used to obtain detailed 3D structures of sub-cellular macro-molecular objects such as supra-molecular multi-protein structures. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. The information is collected and used to assemble a 3-D image of a target. For biological applications, the typical resolution is in the range of 5-20nm.

Cryo-Leica EM AFS2

Cryo-Leica EM AFS2Electron microscopy has a Leica EM AFS2 freeze substitution machine that is used in concert with the the Leica EM PACT2 high pressure freezing machine for cryo electron microscopy.

Cryo-Leica EM PACT2

Cryo-Leica EM PACT2Electron microscopy has a Leica EM PACT2 high pressure freezing machine that is used in concert with the Leica EM AFS2 freeze substitution machine for cryo electron microscopy.