Transmitted Light and Fluorescence Microscopy

Location: Thomas Building, DE-512
Contact phone: (206) 667-4205
Contact e-mail: imaging@fredhutch.org

The Scientific Imaging Shared Resource provides support for both transmitted light and fluorescence microscopy. Transmitted light techniques available in the resource include brightfield, differential interference contrast (DIC, Nomarski), phase contrast, and polarized light microscopy. The resource relies on scientific grade monochrome and/or color microscopy cameras for the acquisition of high resolution digital transmitted light images suitable for publication and quantitative analysis. Instruments recommended for transmitted light microscopy include the Nikon E800, Nikon Live, Tissuefax, and Incucyte. Several other instruments are also capable of imaging in transmitted light mode. For details, follow the links to individual microscopes or services, or contact the resource.

Fluorescence Microscopy

The majority of modern microscopy techniques rely on fluorescence as a means to provide highly specific high contrast labeling of cells and biological molecules. Labeling can be direct (with fluorescent protein fusions, for example), or indirect (for instance with labeled antibodies or nucleic acid probes). Fluorescent techniques are compatible with both fixed and live specimens. All microscopes in the imaging resource are capable of fluorescence imaging. For technical details, use the information found for each instrument in the Instrument dropdown list. To find the microscope best suited to your needs, select compatible dyes and labels, or consult the resource for assistance with experiment planning.

Scheduling time for instruments, training, and support:

To schedule time on any of our microscopes, or to schedule staff assistance or training, please use iLab. The resource is open to all from 9:00 AM - 5:00 PM, Monday through Friday. After hours access can be granted to experienced users and requires training by the resource's staff. Please contact Scientific Imaging for further details.

Please notify the resource if you need to cancel or change a reservation, so that we can make instruments available to others if needed. Users who are more than one hour late without notice may lose their reservation.

How to access your data:

Data acquired on Scientific Imaging instruments is transferred to the si folder in the user's fred account. If you do not have a fred account, please submit the computing account application form. Once you have obtained a fred account, please contact Scientific Imaging so that we can create an si link for you. External users without access to fred can obtain their data via ftp, or can download to their own data storage device from one of our dedicated computers. Please note that no portable drives are allowed on instrument computers.