Nanostring nCounter

Contact: Cassie Sather
Location: Thomas Bldg DE-740
Contact phone: (206) 667-2757
Contact fax: (206) 667-2825
Contact e-mail: csather@fredhutch.org

Introduction

The NanoString nCounter system is an automated system that provides a direct measurement of gene expression using a novel digital color-coded barcode technology.

Up 800 different targets can be analyzed within a single specimen of any species using NanoString’s pre-made CodeSets or a custom designed CodeSet.  The data is quantitative and provides accurate, precise and reproducible results without the need for amplification.  The NanoString Digital Analyzer counts single molecules tagged with unique color barcodes rather than measuring fluorescent intensity, as is done with microarrays.  The system is flexible with the ability to detect RNA, miRNA, DNA, and protein in a variety of different sample types, including:

  • Tissue (fresh, frozen, FFPE-derived total RNA)
  • Crude Cell & Tissue lysates
  • Whole Blood, Other Biological Fluids
  • Total RNA
  • Genomic DNA
  • Single-cells (requires minimal amplification)

For a detailed description of the technology please look at the NanoString website.

Reagents and Consumables

For a list of pre-designed CodeSets please refer to the NanoString website or reach out to our NanoString sales team: