Mycoplasma Testing

Contact: Emil Madraimov
Location: Eastlake Building, E1-305
Contact phone: (206) 667-3756
Contact fax: (206) 667-2892
Contact e-mail: rcb@fredhutch.org

Mycoplasma is a major cause of cell culture contamination and possible loss.  Mycoplasmas are simple, bacteria-like microbes that are the smallest self-replicating organism known (0.3-0.8µm in diameter). They have no cell wall and are fastidious in their growth requirements. This means that they are too small to be seen microscopically, will be colorless in a gram stain, and will not grow on conventional bacteria culture plates, making it a very hard organism to detect in cell cultures.

Mycoplasma assays offered

Rapid Mycoplasma Testing is performed using the MycoProbe kit from R&D systems. This test specifically detects the most common strains of mycoplasma and confirms a positive result with no need to re-test elsewhere. However, it does not detect other bacterial contamination. The turnaround time is up to 5 business days from date of request.

Mycoplasma and Bacteria Testing is performed using the Plasmotest cell based assay  kit from Invivogen. This test detects the presence of any bacteria, including mycoplasma. However if the test is positive, it cannot confirm mycoplasma. This test is run every 2-3 months.

Sample requirements

MycoProbe kit: 100-200ul of culture supernatant. This can be delivered to our lab (E1-305) anytime between 7am-5pm Monday thru Fridays as either fresh or frozen aliquots .The culture should not be fed the day you take the sample (take sample in the morning before feeding); however, there is no need to culture without antibiotics for this kit.

PlasmoTest kit: 100-200ul of culture supernatant. This can be delivered to our lab (E1-305) anytime between 7am-5pm Monday thru Fridays as either fresh or frozen aliquots .The culture should not be fed the day you take the sample (take sample in the morning before feeding) and should be clear of antibiotics for at least 3-5 days.

Doing Business with Specimen Processing/Research Cell Bank

Eastlake Building, E1-305
SPL: (206) 667-4645; RCB: (206) 667-3756

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