Monitoring and Phenotyping Immune Cells

Location: Thomas Building, DE-550
Contact phone: (206) 667-6455
Contact fax: (206) 667-6845
Contact e-mail: jcao@fredhutch.org

Immune monitoring and phenotyping by FACS analysis

Analyzing cell surface and intracellular proteins by labeling with fluorochrome-conjugated antibodies and flow cytometry is a common method in immunology. Flow cytometric analysis can be used to enumerate cell subsets according to their differential markers, activation, functional and other characteristics. Specific cell subsets can be sorted out from a large population of cells using a cell sorter. Antigen-specific T cells can be analyzed after labeling with specific pMHC tetramer. The Immune Monitoring lab provides cellular analysis by flow cytometry in accordance to specific study requirements.

For any of the above assays please use iLab to request the service.