ELISA

Contact: Emil Madraimov
Location: Eastlake Building, E1-305
Contact phone: (206) 667-3756
Contact fax: (206) 667-2892
Contact e-mail: rcb@fredhutch.org

ELISA (enzyme-linked immunosorbent assay) is a plate-based biochemical technique that is used to detect and quantify analytes such as proteins, antibodies, hormones etc. There are a few variations of the assay, but the principle is the same: analyte is bound to the plate surface directly (direct ELISA) or through a capture antibody (indirect or sandwich ELISA). An antibody that is linked to a color-producing enzyme then binds to the analyte. Once the enzyme substrate is added, the reaction results in a color change, which can be measured using a spectrometer.

With experience in performing immunoassays, biological sample handling, high quality standards and hardware capacity (automated microplate washer, spectrophotometers, and automated multichannel pipettors), Research Cell Bank is well equipped to perform ELISA and other plate-based immunoassays. For more information or to discuss the specifics of your ELISA needs, contact us at rcb@fredhutch.org or (206) 667-3756.

Doing Business with Specimen Processing/Research Cell Bank

Eastlake Building, E1-305
SPL: (206) 667-4645; RCB: (206) 667-3756

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