Chimerism Analysis Using Fluorescent VNTR

Location: Thomas Building, D1-315
Contact phone: (206) 667-3369
Contact fax: (206) 667-5978
Contact e-mail: mharkey@fredhutch.org

This technology distinguishes and quantifies the relative contributions of donor and recipient cells in a transplant setting. It can also be used to distinguish and track cells from any two individuals in a chimera or complex cell mixture, either in vivo or in vitro. The method is based on Variable Number Tandem Repeat (VNTR) PCR, which distinguishes alleles of highly polymorphic microsatellite regions of mammalian DNA.

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