Contact: Jeff Delrow
Location: Thomas Bldg DE-740
Contact phone: (206) 667-2714
Contact e-mail: firstname.lastname@example.org
The Bioinformatics Shared Resource is staffed by 3 dedicated bioinformatics specialists and a database developer/programmer.
In an effort to assist researchers with exploring and understanding genomics and proteomics data, bioinformatics support is offered to researchers through two different service models:
Users of the Genomics or Proteomics Shared Resources
Basic analytical support is provided to assist researchers with data generated in either facility. In such cases, up to 4 hours of bioinformatics support is provided free-of-charge. Examples of deliverables are provided below based on the type of assay performed. Additional support is provided for a fee using an hourly rate structure, and is subject to staff availability.
Externally Generated Data
While priority is given to those using the Fred Hutch Genomics and Proteomics Shared Resources to generate data, support is sometimes available to researchers with their own data sets or with data found in publically accessible repositories. In this case, support is provided solely on a fee-for-service basis using an hourly rate structure. Support is subject to staff availability and project demands. Please email email@example.com to discuss the scope of your project and to get an estimate of service fees.
- Filter low quality reads and align to reference genome using TopHat
- Generate QC reports: FastQC and RNA-SeQC
- Associate reads at the gene-level using HTSeq-count
- Detect differential genes using the Bioconductor package edgeR
Whole Exome Sequencing (WGS)
- QC report on all samples submitted (FastQC)
- Alignment using BWA to reference sequence
- Analysis-ready bam files based on Board GATK best practice
- Coverage statistics
- Annotated variant report of SNVs and small Indels in VCF format
- Somatic point mutation using MuTect if paired samples
- QC, align resulting FASTQ files with BWA (or other suitable aligner)
- Identify binding sites with Model-based Analysis for ChIP-Seq (MACS)
- Peak and summit locations in BED-format files.
Proteomics Standard Deliverables
Proteome Discoverer is utilized for processing qualitative and quantitative proteomics data collected on ThermoScientific mass spectrometers. The standard deliverables are:
- Protein identification
- Protein quantitation
- Protein modification analysis
- Pathway analysis