Keith R. Jerome
M.D., Duke University, 1993.
Ph.D., Duke University, Immunology, 1992.
B.S., Georgetown College, Chemistry, 1985.
My research focuses on chronic and latent viruses, their immune evasion mechanisms, and potential curative therapeutic approaches to these infections. Recent work involves the use of DNA editing enzymes such as homing endonucleases and zinc finger nucleases to specifically target latent viral DNA for cleavage and inactivation. This approach may allow precise elimination of functional viral DNA from infected cell reservoirs, offering the prospect of cure for HIV, hepatitis B, herpes simplex, and human papillomavirus infections.
Clinically, I serve as Director of the University of Washington molecular virology laboratory, and consult regarding all aspects of virology testing. Specific areas of interest include real time PCR, automation of nucleic acid extraction, and diagnosis of disease caused by herpesviruses, enterovirus, JC and BK viruses, parvovirus B19, and hepatitis virus.
American Society for Microbiology
Infectious Diseases Society of America
Digital detection of endonuclease mediated gene disruption in the HIV provirus.. Scientific reports. 6:20064.. 2016.
Consistent viral DNA quantification after prolonged storage at ambient temperature.. Journal of virological methods.. 2015.
Impact of rapid influenza PCR testing on hospitalization and antiviral use: A retrospective cohort study.. Journal of medical virology.. 2015.
Inherited chromosomally integrated human herpesvirus 6 as a predisposing risk factor for the development of angina pectoris.. Proceedings of the National Academy of Sciences of the United States of America.. 2015.
Valganciclovir for the prevention of complications of late cytomegalovirus infection after allogeneic hematopoietic cell transplantation: a randomized trial.. Annals of internal medicine. 162(1):1-10.. 2015.
Comparison of the Simplexa HSV1 & 2 Direct kit and laboratory-developed real-time PCR assays for herpes simplex virus detection.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 62:103-5.. 2015.
A patient self-collection method for longitudinal monitoring of respiratory virus infection in solid organ transplant recipients.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 62:98-102.. 2015.
Lack of viral control and development of combination antiretroviral therapy escape mutations in macaques after bone marrow transplantation.. AIDS (London, England). 29(13):1597-606.. 2015.
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.. Journal of medical virology. 88(1):115-119.. 2015.
Evaluation of the Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus.. Journal of clinical microbiology.. 2014.
The potential advantages of digital PCR for clinical virology diagnostics.. Expert review of molecular diagnostics. 14(4):501-7.. 2014.
Human herpesvirus 6 can be detected in cerebrospinal fluid without associated symptoms after allogeneic hematopoietic cell transplantation.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.. 2014.
Past, present, and future perspectives on the diagnosis of Roseolovirus infections.. Current opinion in virology. 9:84-90.. 2014.
In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease.. Molecular therapy. Nucleic acids. 3:e146.. 2014.
Respiratory Syncytial Virus Lower Respiratory Disease in Hematopoietic Cell Transplant Recipients: Viral RNA Detection in Blood, Antiviral Treatment, and Clinical Outcomes.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.. 2013.
Robust suppression of env-SHIV viremia in Macaca nemestrina by 3-drug ART is independent of timing of initiation during chronic infection.. Journal of medical primatology. 42(5):237-46.. 2013.
TALENs targeting HBV: designer endonuclease therapies for viral infections.. Molecular therapy : the journal of the American Society of Gene Therapy. 21(10):1819-20.. 2013.