M.D., Case Western Reserve University, Medicine, 1990.
The Fredicks laboratory develops and applies molecular diagnostic tests for the detection and identification of microbial pathogens, including those pathogens that are difficult to culture in the laboratory. We employ the polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) to detect and localize bacteria and fungi in human tissues. These molecular approaches are applied to improve the detection of well known pathogens, and to investigate the role of novel or uncultivated microbes in unexplained human diseases (pathogen discovery). The laboratory has developed a suite of PCR assays for the detection of fungal pathogens in immunocompromised patients.
We also study the constituents of the human indigenous microbiota, using cultivation-independent approaches to characterize microbes present on human mucosal and epithelial surfaces. The microbiology of the human vagina is an area of research focus, and we are particularly interested in the role of fastidious bacteria in the syndrome bacterial vaginosis. We also study the role of fastidious vaginal bacteria in other adverse health conditions in women.
Previous research has been performed on the cultivation-resistant pathogens Tropheryma whipplei (bacterial cause of Whipple Disease) and Rhinosporidium seeberi (protistan parasite that causes nasal and ocular tumors in rhinosporidiosis).
American Association for the Advancement of Science
American Society for Microbiology
Infectious Diseases Society of America
Honors and Awards
Physician Scientist Award, National Institutes of Health, Stanford University
Program Commitee Choice Award, Infectious Diseases Society of America, Best abstract at 2004 annual meeting, Boston.
James McDougall Faculty Mentor Award, Fred Hutchinson Cancer Research Center
2007-2013, Associate Professor, University of Washington, School of Medicine, Allergy, and Infectious Diseases
2007-2012, Associate Member, Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division & Clinical Research Division
2001-2007, Assistant Member, Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Infectious Diseases
2001-2007, Assistant Professor, University of Washington, School of Medicine, Medicine, Allergy and Infectious Diseases
1998-2001, Research Associate, Stanford University, School of Medicine, Medicine, Infectious Diseases
Disseminated coccidioidomycosis presenting with intramedullary spinal cord abscesses: Management challenges.. Medical mycology case reports. 15:1-4.. 2017.
Semen Bacterial Concentrations and HIV-1 RNA Shedding among HIV-1-Seropositive Kenyan Men.. Journal of acquired immune deficiency syndromes (1999).. 2016.
Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis.. The Lancet. Infectious diseases.. 2016.
More Easily Cultivated Than Identified: Classical Isolation With Molecular Identification of Vaginal Bacteria.. The Journal of infectious diseases. 214 Suppl 1:S21-8.. 2016.
Changes in Vaginal Microbiota and Immune Mediators in HIV-1-Seronegative Kenyan Women Initiating Depot Medroxyprogesterone Acetate.. Journal of acquired immune deficiency syndromes (1999). 71(4):359-66.. 2016.
Relationship of Specific Bacteria in the Cervical and Vaginal Microbiotas With Cervicitis.. Sexually transmitted diseases. 42(9):475-81.. 2015.
Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women.. American journal of obstetrics and gynecology. 212(5):611.e1-9.. 2015.
First Trimester Levels of BV-Associated Bacteria and Risk of Miscarriage Among Women Early in Pregnancy.. Maternal and child health journal.. 2015.
Rapid and Profound Shifts in the Vaginal Microbiota Following Antibiotic Treatment for Bacterial Vaginosis.. The Journal of infectious diseases.. 2015.
Correlation between Circulating Fungal Biomarkers and Clinical Outcome in Invasive Aspergillosis.. PloS one. 10(6):e0129022.. 2015.
Metabolic signatures of bacterial vaginosis.. mBio. 6(2). 2015.
Early pregnancy changes in bacterial vaginosis-associated bacteria and preterm delivery.. Paediatric and perinatal epidemiology. 28(2):88-96.. 2014.
Voriconazole therapeutic drug monitoring: retrospective cohort study of the relationship to clinical outcomes and adverse events.. BMC infectious diseases. 13(1):105.. 2013.
Associations between vaginal bacteria and levels of vaginal defensins in pregnant women.. American journal of obstetrics and gynecology. 208(2):132e.1-7.. 2013.
Bacterial vaginosis-associated bacteria in men: association of Leptotrichia/Sneathia spp. with nongonococcal urethritis.. Sexually transmitted diseases. 40(12):944-9.. 2013.
Extravaginal reservoirs of vaginal bacteria as risk factors for incident bacterial vaginosis.. The Journal of infectious diseases. 205(10):1580-8.. 2012.
Effect of sexual debut on vaginal microbiota in a cohort of young women.. Obstetrics and gynecology. 120(6):1306-13.. 2012.
Enhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy.. Medical mycology : official publication of the International Society for Human and Animal Mycology. 50(6):667-72.. 2012.