High Pressure Freezing Symposium
Tuesday, March 21, 2017
Please join us for the lecture and demos on March 21st, starting at 9 am at Fred Hutch at 1100 Fairview Ave North, Seattle WA 98109. Everybody is welcome to attend lecture! Please contact the EM Resource Staff at 206-667-4289 to schedule time to demo the machine or freeze your samples.
New Developments in High Pressure Freezing
Adding Motion and Color to Electron Micrographs
Electrical and Light Stimulation!
Shigeki Watanabe, Ph.D. - Johns Hopkins University School of Medicine
Location: Weintraub Building, Room B1-072/074 at 9 am
Electron micrographs depict subcellular organization of a cell but often lack temporal and molecular information. What is the cell doing? Where are proteins in the cell? To overcome these limitations, I have developed two novel techniques: “flash-and-freeze” and nano-resolution fluorescence electron microscopy. “Flash-and-freeze” adds temporal information by stimulating physiological changes in neurons with channel rhodopsin and capturing the subsequent cellular dynamics with high-pressure freezing. Nano-resolution fluorescence electron microscopy adds molecular information to electron micrographs by coupling super-resolution fluorescence imaging with electron microscopy. I am using these two techniques to study membrane and molecular dynamics of synapses.
Tuesday, March 21 Schedule
8:45 am – Greetings and Coffee
9:00 am – Lecture on High Pressure Freezing, Weintraub Building, B1-072/074
10:00 am – 5 Leica ICE HPF demo and sample runs
EM Resource, Thomas Building, DE-780
If you are interested in attending, please register with:
Bobbie Schneider, EM Director
Fred Hutchinson Cancer Research Center
Electron Microscopy Resource, DE-780
1100 Fairview Ave. N.
Seattle, WA 98109
All visitors must sign in and record their vehicle information at the Thomas building reception desk, the Yale building Security and Transportation desk or the Arnold building front desk. Fred Hutch and Seattle Cancer Care Alliance employees are not permitted to park in visitor parking.
For more parking info please go to: https://www.fredhutch.org/en/contact-us/visit-us/parking.html
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